COMPOSITION OF ESSENTIAL OIL FROM DRIED BASIL (Ocimum Basilicum L.)

Mambetsheripova A.A

1Karakalpak state university named after Berdakh

Sultanova Sh.A2

2Deputy mayor of Tashkent city

Safarov J.E3

3Tashkent state technical university

Keywords: essential oils, basil, hydrodistillation, gas chromato-mass spectrometry (GC-MS), component composition, yield, aromatic compounds.


Abstract

The present work describes a methodological approach for the isolation and analysis of essential oils (EOs) obtained from finely ground basil plants. The extraction of basil EO was carried out by hydrodistillation using a classical Clevenger apparatus for a duration of three hours. The distillates were subsequently treated with n-hexane, followed by dehydration with anhydrous sodium sulfate in order to remove residual moisture. The yield of the essential oil under these conditions varied within the range of 0.74–0.81%. The isolated oils were characterized as pale-yellow, mobile liquids possessing a specific aromatic odor typical of basil. For the identification of chemical constituents, the qualitative and quantitative composition of the oils was studied using gas chromatography–mass spectrometry (GC–MS). The analyses were performed on an Agilent 5975C inert MSD/7890A GC instrument equipped with a quartz capillary column Agilent HP-INNOWax, under a programmed temperature regime. This integrated approach enables reliable isolation, precise characterization, and effective identification of the component profile of basil essential oils.


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