Multidisciplinary Journal of Science and Technology

EFFECT OF PROBIOTICS ON ZONULIN LEVELS AND INTESTINAL MICROBIOTA COMPOSITION IN FUNCTIONAL GASTROINTESTINAL DISORDERS

Yarashbayeva Asal

1st year master’s student in internal diseases ,Urgench state medical institute, Urgench, Uzbekistan

Keywords: functional gastrointestinal disorders, zonulin, intestinal permeability, gut microbiota, probiotics

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Abstract

Functional gastrointestinal disorders (FGIDs) are among the most prevalent chronic conditions in gastroenterological practice and are characterized by persistent gastrointestinal symptoms in the absence of identifiable structural pathology. Increasing evidence indicates that impaired intestinal barrier function and alterations in gut microbiota composition play a central role in the pathogenesis of FGIDs. Zonulin, a key regulator of intestinal tight junctions, serves as a biomarker of increased intestinal permeability and is frequently elevated in patients with FGIDs.

This study was conducted at the Khorezm regional multidisciplinary medical center and aimed to evaluate the effect of probiotic therapy on serum zonulin levels and intestinal microbiota composition in patients with functional gastrointestinal disorders. Patients were stratified into study groups based on treatment modality. The results demonstrate that probiotic supplementation significantly reduces zonulin levels, improves gut microbiota balance, and alleviates clinical symptoms, confirming the role of probiotics as an effective adjunct in FGID management.

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References

1. Drossman D.A. Functional gastrointestinal disorders: history, pathophysiology, clinical features, and Rome IV. Gastroenterology. 2016;150(6):1262–1279.

2. Sperber A.D., Bangdiwala S.I., Drossman D.A., et al. Worldwide prevalence and burden of functional gastrointestinal disorders: results of the Rome Foundation global study. Gastroenterology. 2021;160(1):99–114.

3. Mayer E.A., Tillisch K., Gupta A. Gut/brain axis and the microbiota. J Clin Invest. 2015;125(3):926–938.

4. Camilleri M. Leaky gut: mechanisms, measurement and clinical implications in humans. Gut. 2019;68(8):1516–1526.

5. Fasano A. Zonulin, regulation of tight junctions, and autoimmune diseases. Ann N Y Acad Sci. 2012;1258:25–33.

6. Sapone A., de Magistris L., Pietzak M., et al. Zonulin upregulation is associated with increased gut permeability in subjects with functional gastrointestinal disorders. Gut. 2011;60(7):998–1004.

7. Ohlsson B., Orho-Melander M., Nilsson P.M. Higher levels of serum zonulin may rather be associated with increased risk of obesity and hyperlipidemia than with gastrointestinal symptoms. United European Gastroenterol J. 2017;5(3):357–363.

8. Shreiner A.B., Kao J.Y., Young V.B. The gut microbiome in health and in disease. Curr Opin Gastroenterol. 2015;31(1):69–75.

9. Jeffery I.B., O’Toole P.W. Diet–microbiota interactions and their implications for healthy living. Nutrients. 2013;5(1):234–252.

10. Hill C., Guarner F., Reid G., et al. Expert consensus document: The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat Rev Gastroenterol Hepatol. 2014;11(8):506–514.

11. Plaza-Díaz J., Ruiz-Ojeda F.J., Gil-Campos M., Gil A. Mechanisms of action of probiotics. Adv Nutr. 2019;10(Suppl 1):S49–S66.

12. Barbara G., Grover M., Bercik P., et al. Rome Foundation working team report on gut microbiota and the gut–brain axis in functional gastrointestinal disorders. Gastroenterology. 2019;156(5):1410–1425.

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